Статья: Morphological and Functional Characteristics of the Heart under Chronic Ischemic Injury Conditions Following the Use of Allogeneic Biomaterial in Rats (2025)

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One of the scientific approaches to correcting fibrotic myocardium is the development of regenerative medicine methods, which involve tissue therapy. The present study aimed to identify morphological and functional changes in the fibrous myocardium of rats after cryoinjury and the use of allogeneic biomaterial. The study included 80 Wistar rats divided into two groups, the control and the experimental group. All rats underwent a thoracotomy under general anesthesia with Xylazine and Zolazepam, and a section of the left ventricle was frozen with a cooled metal stylet. In the experimental group, a repeated thoracotomy was performed 45 days after cryodestruction. An allogeneic biomaterial suspension, dispersed from an acellular extracellular matrix with a particle size of 50 to 80 μm, was then injected intracardially into the area of cryogenic myocardial necrosis at a dose of 3 mg per animal. Forty-five days after the allogenic biomaterial injection, the tolerance of rats to physical activity was assessed using the modified Porsolt test. After the tolerance test, the weight of the heart, the thickness of the left ventricular wall, the thickness of the scar, and the diameter of the damaged area were measured. Three myocardium samples were taken from each animal and processed for examination using a light microscope. Histological examination of the myocardium samples revealed an avascular scar. In the experimental group, the thickness of the muscular part was significantly higher than in the control group. Meanwhile, heart weight didn’t differ much between the control and experimental group. The exercise tolerance of rats in the experimental group was higher than that of the control group and did not differ significantly from the intact values. The presented results supported the allogeneic biomaterial’s positive influence on the rats’ myocardium structure that had undergone cryogenic destruction.

Ключевые фразы: allogeneic biomaterial, chronic ischemic injury, cryodestruction, heart, myocardium

Автор (ы): Lebedeva Anna, Musina Lyalya, Prusakov Alexey, Ponamarev Vladimir

Журнал: WORLD'S VETERINARY JOURNAL

Предпросмотр статьи

Идентификаторы и классификаторы

УДК: 57. Биологические науки

Для цитирования:

LEBEDEVA A., MUSINA L., PRUSAKOV A., PONAMAREV V. MORPHOLOGICAL AND FUNCTIONAL CHARACTERISTICS OF THE HEART UNDER CHRONIC ISCHEMIC INJURY CONDITIONS FOLLOWING THE USE OF ALLOGENEIC BIOMATERIAL IN RATS // WORLD'S VETERINARY JOURNAL. 2025. № 2, ТОМ 15

Текстовый фрагмент статьи

Even with advancements in diagnostic and treatment technologies such as CT scans, MRI, and ultrasound, chronic ischemic heart disease continues to be a major cause of cardiovascular disease and mortality globally (Fácila Rubio et al., 2024). Ischemic myocardial pathologies, such as Ischemic cardiomyopathy, myocardial infarction, and angina pectoris in animals, as in humans, are associated with insufficient blood supply to the heart muscle (Algoet et al., 2023). For pharmaco-correction of such conditions, Nitrates (Nitroglycerin) are used to dilate blood vessels and improve blood flow, beta-blockers (Atenolol) reduce heart rate and reduce the load on the heart, antiplatelet agents (Aspirin) and anticoagulants (Warfarin) prevent blood clots, and angiotensin-converting enzyme inhibitors (Enalapril) lower blood pressure and reduce the load on the heart (Chen et al., 2024). Despite significant breakthroughs in cardiology, the search for effective methods to restore cardiac function after ischemic injury remains an urgent task. About 5 to 15% of patients discharged from the hospital die within the first year; the subsequent annual mortality rate ranges from 2 to 5% (Baryshev et al., 2022; Kuznetsov et al., 2022; Alekhin et al., 2023). The high medical and social significance of myocardial infarction underscores the need for further improvement and development of effective and affordable treatment methods in the long-term management of the disease to prevent fibrotic degeneration of the myocardium, which impairs heart function (Garganeeva et al., 2015). One of the scientific approaches to correcting fibrotic myocardium can be the development of regenerative medicine methods, such as tissue therapy (Ponamarev et al., 2023a, b; Prusakova et al., 2023a, b). Recently, increasing attention has been paid to the use of biomaterials for regenerating damaged tissues. In particular, Allogeneic biomaterial (AB) represents a promising direction in cardiology due to its biocompatibility and potential to stimulate regenerative processes (Kruglyakov et al., 2004; Afanasiev et al., 2009; Garganeeva et al., 2015). The AB, derived from the acellular extracellular matrix, has proven to be a stimulator of organ and tissue regeneration, but it has not been previously used in cardiology (Muldashev et al., 2005).

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Выпуск

№ 2, Том 15 (2025)

Кол-во страниц: 8 страниц

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