Научная статья: Characterization of the unusual PII protein with elongated T-loop from Micromonas pusilla (2025) (читать, скачать)

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The PII superfamily consists of widespread signal transduction proteins found in all domains of life. The most conserved PII-interactor across oxygenic phototrophs from cyanobacteria to Archaeplastida is the key enzyme of the ornithine/arginine synthesis pathway, N-acetyl-L-glutamate kinase (NAGK). T-loops represent the major PII-receptor binding element and are involved in the interaction with NAGK. Within the class Mamiellophyceae, only the genus Micromonas contains species with the PII protein. Bioinformatic analysis revealed that the PII protein of Micromonas pusilla (MpPII) has an unusually prolonged T-loop. Here, we performed the coupled enzyme assay and showed that MpPII has no remarkable influence on NAGK activity. An engineered variant of MpPII with deletion of four additional amino acids (AATD) in the T-loop restored the ability of this protein to relieve NAGK from feedback inhibition by arginine in a glutamine-dependent manner. The findings are discussed in the context of unusual plasticity of the PII protein family during the evolution of Archaeplastida.

Ключевые фразы: mamiellophyceae, micromonas pusilla, n-acetyl-l-glutamate kinase, pii proteins

Автор (ы): VLASOVA V., LAPINA T., ZALUTSKAYA ZH., CHENG QI., ERMILOVA E.

Журнал: PROTISTOLOGY

Предпросмотр статьи

Идентификаторы и классификаторы

SCI: Биология
УДК: 57. Биологические науки

Для цитирования:

VLASOVA V., LAPINA T., ZALUTSKAYA ZH., CHENG QI., ERMILOVA E. CHARACTERIZATION OF THE UNUSUAL PII PROTEIN WITH ELONGATED T-LOOP FROM MICROMONAS PUSILLA // PROTISTOLOGY. 2025. № 1, ТОМ 19

Текстовый фрагмент статьи

Список литературы

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Выпуск

№ 1, Том 19 (2025)

Кол-во страниц: 65 страниц

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Статья: Characterization of the unusual PII protein with elongated T-loop from Micromonas pusilla (2025)